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KMID : 0900919940180010055
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Korean journal of Animal Reproduction
1994 Volume.18 No. 1 p.55 ~ p.62
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Effects of FDA-Test on the Survival and Conception Rate in Vitrified Mouse Embryos I. Effects of Addition Levels of FDA(Florescein Diacetate) on Survival and Conception Rate in Vitrified Mouse Morulae
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Moon Sung-Ho
Kang Min-Soo
Jang Deok-Jee
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Abstract
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This study was carried out to test the effects of fluorescein diacetate(FDA 0{mu}ell/ml, 0.5{mu}ell/ml, 5{mu}ell/ml, 10{mu}ell/ml or 0{mu}ell/ml, 5{mu}ell/ml in PBS) treated before culture on the survival of vitrified mouse morulae in vitrification solution(20% glycerol＋glycerol＋10% ethylene glycol＋30% ficoll＋10% sucrose). The results were summarized as follows; 1. The survival rate of FDA-tested fresh mouse morulae after 24 hours culture was over 96%((P4.8) in the control or treatment groups with various levels of FDA. Because the rate of mouse morulae which developed to hatched blastocysts was higher with the various levels of FDA treatment(67%) than control(50%), it was considered that toxicity of FDA did not affect the survival of mouse morulae. 2. When mouse morulae were FDA-tested in FDA 0(control), 0.5, 5, and 10{mu}ell/ml treatment after vitrification, the development rate to expanded blastocyst were 66, 82, 64 and 76%, and FDA scores were P4.2(84%), P4.7(94%), P4.2(84%) and P3.9(78%), respectively. There were no significant differences between control and FDA treatments, but there were significant difference between 0.5{mu}ell/ml)94%) and 10{mu}ell/ml(78%) treatment(P<0.01). 3. The survival rates of cultured mouse morulae according to FDA-scores(P0=non-fluorescence; P1~P5=according to their fluorescence) after vitrification were P5;92%, P4;67%, P3;42% and P2.P0;0%, respectively. 4. Implantation rates of morulae stage embryos cultured into early blastocysts and implanted into uterine hornes vitrification were 14 and 11% embryos treated control(0{mu}ell/ml) and FDA 5{mu}ell/ml and the normal fetus development was 2% embryos for both treatments. Results of this percent study indicated that toxicity of FDA does not affect not only the survival of fresh and vitrified mouse morulae but also the development rate and implantation of fetus after transfer as well. The development rates of mouse morulae with the FDA score of P5, P4 and P3 were 92, 67 and 42%, respectively, it was considered that FDA-test was fit for the judge of survival.
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KEYWORD
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Vitrification solution, Mouse morula, Transfer, FDA-test, Expanded blastocyst, Implantation
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